Human Alpha-Aminoadipic Semialdehyde Synthase (AASS)The content of this page was edited by SGC and matteoferla on the 2019-10-07 15:59:04.949409.
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This work provides the early tools to develop substrate reduction inhibitors for a genetic childhood seizure disorder, with the hypothesis to target the enzyme (AASS) upstream of the defective gene (ALDH7A1) in the human lysine metabolic pathway. This TEP package includes recombinant human AASS purification protocols, structures of the AASS second domain in different states, in vitro assays to detect ligand binding (differential scanning fluorimetry) and enzyme activity (NADH formation) of human AASS, as well as initial chemical matters.
Structures available in TEP:
- Structure of hAASS-SDH apo (PDB:5O1P ): ,
- Structure of hAASS-SDH bound with NAD (PDB:5L78 ): ,
- Structure of hAASS-SDH bound with Pro (PDB:5O1O ): ,
- Structure of hAASS-SDH bound with N-[(2S)-2-Pyrrolidinylmethyl]-trifluoromethanesulfonamide (compound 2) (PDB:5O1N ): ,